About this manual. This manual describes the operation of the ÄKTA™FPLC™ system. System description, system maintenance and trouble-shooting are also. filter. Sample pump. Buffer select valve (V6). Injection valve (V1). Reverse . Manual. Flowpath. B1 or B2. BufferValveB. Pump A. Pump B. Common inlet. A2. A1. About this manual This manual describes the operation of ÄKTA purifier: Evaluating the UV response Calculate the UV response ratios in the following.

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Click Help in the dialog for more information on the options in the Method Wizard.

Hardware items, for example, keyboard key names, key combinations, key sequences, hard key names on the equipment and connector port names located on the equipment are identified in the text by bold text. Examples on peak fractionation parameters are: Akfa is achieved by using the following method: The valve should be connected to the UniNet-2 socket.

This is described in the respective component manuals. Store all the boxes and plastic bags in a convenient nearby place. Check that the correct mixer volume is used. You can repeat this for other curves. Check that the pump is operating correctly. The modules and components contain high voltage circuits that can give a lethal electric shock.

If not, purge the pump with a syringe as described in 2. Five loop volumes ensure better precision. Check the Show details box to view them.


ÄKTApurifier User Guide – GE Healthcare Life Sciences |

If any of the evaluated values falls outside the specified range, go to Section 2. Optical unit If not, set the wavelength by selecting lamp position indicated by a dot on the lamp housing in combination with the appropriate filter the dot on the lamp housing should be adjacent to the manal on the filter housing corresponding to the symbol on the filter wheel for the desired filter.

If you do not select System: For more detailed information, refer to Pump P User Manual. Installation 1 Unpack and assemble Frac according to the instructions given in the Fraction collector Frac User Manual.

ÄKTApurifier – GE Healthcare Life Sciences

If tubing with smaller inner diameter is used, there is a risk that the back-pressure will be too high and the columns might rupture. The illustration to the right shows setting of the wavelength nm. You can zoom further on the enlarged part.

Lay the system on the same side as on the pallet. The UPC offers fixed wavelengths of Zn lampor nm Hg lampfast manuall, high accuracy and reproducibility and flow cells with low dead volumes. The new correction factors will only apply while this method is used. If is not within the limit, replace or contact GE Healthcare. Please add items to your Cart for the correct pricing.

A 5 mm cell is also included in the package. Attach the column holder enclosed in Box above the UV cell.


Waste tubing marked W3 extended to waste reservoir. Note that the term chromatogram is used here when talking about the whole window containing all the different curves.


Low eluent flow and noise as the piston moves 1 Replace or re-install the seal or membrane. For continued protection against risk of fire, replace only with a fuse of the specified type and rating.

The table below describes the three PID parameters. The four stock solutions consist of: Click the Variables page. The mixer function can also be checked by running the installation test.

If the column pressure is higher, the purifidr must be reduced in order not to exceed the pressure limit. Additional buttons are provided in the toolbar. By observing the piston status indicator in the Check menu together with the pressure trace, the pump cylinder that is functioning abnormally can be identified, see Reference information in purlfier Pump P User Manual.

ÄKTApurifier User Guide – GE Healthcare Life Sciences

The table below lists the theoretical volumes of a number of components and tubing. Put the open end in purfier waste container. If the syringe is taken out before the sample is injected onto the column, self-drainage can occur and the loop will be emptied. The fractionation order can be selected as serpentine-row, row-by-row, serpentine-column or column-by-column.